課程介紹
利用獸醫細胞免疫學及染色體基因毒理學配合分子診斷技術,探討疾病及毒物之細胞之免疫及染色體變異分子作用機制,作為探討疾病及中毒發生,加強學生對毒物及疾病之認知,導引學生研究思考方向。
教科書:
Preston, R., and Hoffinann. 2006. Genetic toxicology. In: Klassenn, C.D., Ed., Casarett & Doull’s Toxicology. The Basic Science of Poisons, 6th Edition, Unit 3. p. 321-350.McGwraw-Hill, Medical Publishing Division, New York. USA.
2. Department of Health. 1999. In vitro Chromosomal Aberration Test with Mammalian Cells. In: Genotoxicity study of health foods safety and function. DOH code number: 88037803. Taipei, Taiwan.
3. Detrick . 2006. Manual of molecular & clinical laboratory immunology, 7th Ed. Elsevier.
4. Jacques Descotes. 2004. Principle and methods of immunotoxicology. V1.
5. USEPA. 1998. Mammalian Erythrocyte Micronucleus Test. In: OPPTS Harmonized Test Guidelines, Series 870.5395, EPA 712-C-98-226. 10 pp.
6. USEPA. 1998. In vitro Mammalian Chromosome Aberration Test. In: Health Effects Test Guidelines, OPPTS Harmonized Test Guidelines, Series 870.5375, EPA 712-C-98-223, p. 1-13.教學進度:
- 免疫診斷技術 (Methods for immuno diagnosis)
- 細胞染色體變異之診斷技術原理 (Principals for chromosomal aberration)
- 細胞免疫模式之應用 (Applications for cellular immunological models)
- 細胞染色體變異試驗操作技術 (Protocols for chromosomal aberration)
- 細胞免疫操作技術 (Techniques for cellular immunology)
- 細胞染色體變異之判讀 (Interpretation for chromosomal aberration)
- 細胞免疫作用機制之探討 (Mechanisms for cellular immunology)
- 最後指導研究報告之撰寫及綜合討論(Research report writing and discussion)
本課程不開放報名